UNIVERSITY OF WISCONSIN River Falls

Lisa Kroutil
Lisa Kroutil

Lisa Kroutil

Associate Professor
member of graduate faculty

Chemistry
Office: 261 Centennial Science Hall
Phone: 715-425-3577

Email: lisa.c.kroutil@uwrf.edu

Education:

B.S. University of Missouri - Rolla, 1988 
Ph.D. Washington University, 1994 
Postdoctoral Fellow National Institute of Environmental Health Sciences, 1994-1997

Courses Taught:

  • Chemistry 120 - Introductory Chemistry - Lecture and Laboratory
  • Chemistry 121 - General Chemistry I - Lecture and Laboratory
  • Chemistry 122 - General Chemistry II - Lecture and Laboratory
  • Chemistry 361 - Biochemistry I
  • Chemistry 362 - Biochemistry II
  • Chemistry 366 - Biochemistry Lab

Research Interests:

Hairpin GroupResearch Students/Projects:
  • David Olson Chemistry 295 (Fall 2005)
  • Jason Malisheski Chemistry 295 (Fall 2003).
  • Brian Riley performed gap filling synthesis and fidelity studies with Klenow fragment on substrates containing secondary structure (Summer 2002).
  • Jessica Maher continued the work started by Brina Riley (Fall 2002).
  • Jacquie Kvass performed gap filling synthesis and fidelity studies with T7 DNA polymerase and polymerase eta on triplet repeat substrates. (Summer 2002 - Spring 2003).
  • Katie Clay worked on sequencing DNA for use in a trinucleotide repeat insertion assay (Summer 2002).
  • Christa Nichols initiated studies of the thermal stability of a hairpin containing oligonucleotide (Summer 2002).
  • Clayton Carlson prepared gapped DNA substrates for fidelity studies. (supported by Cottrell College Science Award.)
  • Kurt Fleschner examined the fidelity of T7 DNA pol on substrates containing secondary structure. (Supported by a Cottrell College Science Award.)
  • Melissa Ludack attempted to develop a nonradioactive assay for DNA polymerase. (McNair Scholar)
  • Mariah Scott used site-directed mutageneis to prepare an additional DNA sequence for study. (Supported by a Cottrell College Science Award.)
  • Polymerase GroupLiz Walz tried to purify a DNA polymerase. (Supported by an UWRF Faculty Research Grant.)
  • Renee Silvis (Spring 1999, Fall 1999 and Spring 2000) and Melissa Ludack (Spring 2000) started to prepare gapped DNA substrates for fidelity studies.
  • Chad White (Summer 1999) performed research on replication fidelity in sequences capable of forming secondary structures.
  • Dan Robinson (Summer 1999, Fall 1999 and Spring 2000) and Andrea Oestreich (Spring 2000 and Summer 2000) isolated bacteriophage DNA for preparation of gapped substrates.
  • Kendie Runge (Fall 1999) successfully sequenced a section of bacteriophage DNA using Promega's Silver Sequencing protocol.
  • Mulugeta Mamo (Fall 1999 and Spring 2000) and Melissa Meier (Spring 2000) worked on developing a nonradioactive gel assay for polymerase activity and purifying eukaryotic DNA polymerases.
  • Angie Strack and John Goodell tried to synthesize and characterize polyglutamine (Spring 2000).
  • Kim Barrett (Summer 2000) and Andrea Oestreich (Summer 2000, McNair Scholar) worked on the optimization of the Promega Silver Sequencing procedure for our ss-DNA samples.
  • Jason Schmidt and Andrea Oestreich (Summer 2000) prepared gapped substrates containing DNA hairpins.

Background:

I am a biochemist and the director of the interdepartmental Biotechnology Program. I teach introductory chemistry and biochemistry. My research focuses on understanding the processes that lead to replication errors in repetitive and structured DNA sequences.