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Dr. Lisa C. Kroutil
Associate
Professor
University of Wisconsin-River Falls
410 S. Third St.
River Falls, WI 54022
(715) 425-3577
lisa.c.kroutil@uwrf.edu
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Background:
I am a biochemist and the director of the interdepartmental Biotechnology Program. I teach introductory
chemistry and biochemistry. My research focuses on understanding
the processes that lead to replication errors in repetitive and structured
DNA sequences.
Education and Training:
B.S. University of Missouri - Rolla, 1988
Ph.D. Washington University, 1994
Postdoctoral Fellow National Institute of Environmental Health Sciences,
1994-1997
Courses that I teach on a regular basis:
- Chemistry 120 - Introductory Chemistry - Lecture and Laboratory
- Chemistry 121 - General Chemistry I - Lecture and Laboratory
- Chemistry 122 - General Chemistry II - Lecture and Laboratory
- Chemistry 361 - Biochemistry I
- Chemistry 362 - Biochemistry II
- Chemistry 366 - Biochemistry Lab
Research Students/Projects:
- David Olson Chemistry 295 (Fall 2005)
- Jason Malisheski Chemistry 295 (Fall 2003).
- Brian Riley performed gap filling synthesis and fidelity
studies with Klenow fragment on substrates containing secondary structure
(Summer 2002).
- Jessica Maher continued the work started by Brina Riley (Fall
2002).
- Jacquie Kvass performed gap filling synthesis and fidelity
studies with T7 DNA polymerase and polymerase eta on triplet repeat
substrates. (Summer 2002 - Spring 2003).
- Katie Clay worked on sequencing DNA for use in a trinucleotide
repeat insertion assay (Summer 2002).
- Christa Nichols initiated studies of the thermal stability
of a hairpin containing oligonucleotide (Summer 2002).
- Clayton Carlson prepared gapped DNA substrates for fidelity
studies. (supported by Cottrell College Science Award.)
- Kurt Fleschner examined the fidelity of T7 DNA pol on substrates
containing secondary structure. (Supported by a Cottrell College Science
Award.)
- Melissa Ludack attempted to develope a nonradioactive assay
for DNA polymerase. (McNair Scholar)
- Mariah Scott used site-directed mutageneis to prepare an
additional DNA sequence for study. (Supported by a Cottrell College
Science Award.)
- Liz Walz tried to purify a DNA polymerase. (Supported by
an UWRF Faculty Research Grant.)
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DNA
Hairpin Group |
Polymerase
assay and purification group |
- Renee Silvis (Spring 1999, Fall 1999 and Spring 2000) and
Melissa Ludack (Spring 2000) started to prepare gapped DNA
substrates for fidelity studies.
- Chad White (Summer 1999) performed research on replication
fidelity in sequences capable of forming secondary structures.
- Dan Robinson (Summer 1999, Fall 1999 and Spring 2000) and
Andrea Oestreich (Spring 2000 and Summer 2000) isolated bacteriophage
DNA for preparation of gapped substrates.
- Kendie Runge (Fall 1999) successfully sequenced a section
of bacteriophage DNA using Promega's Silver Sequencing protocol.
- Mulugeta Mamo (Fall 1999 and Spring 2000) and Melissa
Meier (Spring 2000) worked on developing a nonradioactive gel
assay for polymerase activity and purifying eukaryotic DNA polymerases.
- Angie Strack and John Goodell tried to synthesize
and characterize polyglutamine (Spring 2000).
- Kim Barrett (Summer 2000) and Andrea Oestreich (Summer
2000, McNair Scholar) worked on the optimization of the Promega Silver
Sequencing procedure for our ss-DNA samples.
- Jason Schmidt and Andrea Oestreich (Summer 2000) prepared
gapped substrates containing DNA hairpins.
