Note: Where the instructions tell you to type a command, only type what is within the quote marks, do not type the quote marks.
1. Follow the initial steps of sample preparation, logging on, locking and shimming, that are used for a routine proton experiment.
2. Be sure that the probe is tuned to 1H.
1H REFERENCE SPECTRUM
3. Run a normal 1D proton spectrum. Examine the spectral width. If desired, reset sw and sfo1 and run again. Write down the name of the data set.
4. When finished, type sw and sfo1 and write down these displayed values.
5. Use the cursor to identify a reference peak and write down the value of this peak.
6. Pull down "output", move to "show-define..." and release on "retain cx".
RUNNING THE 2D EXPERIMENT
7. Turn the spinner off.
8. Pull down the "File" menu, move to "open" and release on "data set owners". Click on "nmr". Pull down the "file" menu, move to "open" and release on "data sets by name". Click on "COSY525". Old data may be displayed. Ignore it.
9. Pull down the "file" menu, move to "new" and release. Click in the box after "user" and type in your owner name. You may also enter a new name for your sample if you choose. Then click on "save".
10. Type "eda" (return). Find SW and click in the box under F2 and enter the SW value for your reference 1H spectrum. Then enter the same value for the SW under F1. Click on "save".
11. Type "sfo1" (return) and enter the sfo1 for your 1H spectrum. (return).
12. Type "sfo2" (return) and enter the sfo1 for your 1H spectrum. (return).
13. Type "rga" (return)
14. Type "zg" (return).
PREPARING FOR PLOTTING
15. Type "edg". Click on "EDPROJ1". Find PF1NAME and enter the name of the 1H dataset and user name that you recorded earlier. Click on "save".
Click on "EDPROJ2". Find PF2NAME and enter the name of the 1H dataset and user name that you recorded earlier. Click on "save" and again on "save" to exit "edg".
PROCESSING
16. Type "xfb" (return). The 2D spectrum will be displayed.
17. Use the "*2" and "/2" buttons to adjust the amplitude to display the spectrum appropriately.
18. Click on "calibrate" and obtain cross hairs on the screen. Move the cross point to a point on the diagonal that coresponds to the reference value on each spectrum that you recorded earlier. Click the center mouse button and enter the reference values.
19. Pull down from "output", move to "define-show..." and release on "current screen limits". Respond to the displayed values with three returns.
20. You can type "setti" in order to put a title on the spectrum, but the title may get written on top of the horizontal 1H spectrum projection.
21. Type "view" (return) to obtain a preview of the plot. (Quit.)
22. Type "plot" (return) to plot.
LOGGING OUT
Log out in the usual manner.
IMPORTANT: Put a standard sample back into the spinner and release the lift button on the keypad. You may also turn on the spinner. Do not leave the magnet with the lift air on and no sample in.
Created by: d.b.rusterholz@uwrf.edu