BIOL 707 Ð
Biotechnology Workshop for Teachers
University of Wisconsin-River Falls
July 7-25, 2003
Rm. 217 Ag-Science
Instructors: Karen Klyczek Scott Ballantyne Tim Buttles
Biology Biology Agriculture Education
UW-River Falls UW-River Falls UW-River Falls
Ph 715-425-3591 Ph 715-425-3362 Ph 715-425-3555
karen.k.klyczek@uwrf.edu scott.m.ballantyne@uwrf.edu timothy.j.buttles@uwrf.edu
Web site: http://www.uwrf.edu/biotech/workshop/welcome.html
Catalog Course Description:
BIOL 707, 4 cr.
This course will illustrate basic
concepts in biology used in biotechnology applications, and how these
applications can be incorporated into science classes. Hands-on lab activities include DNA
isolation and fingerprinting, plant tissue culture, genetic engineering of
bacteria, and fermentation of food. Labs include strategies for making
demonstration-type labs more inquiry-based. Ethical and social issues raised by
the use of these technologies will be illustrated using case studies and
decision-making models.
Core concepts covered: DNA structure and function Protein structure and function
DNA cloning and analysis Cell metabolism and fermentation
Genetic inheritance, chromosomes Mitosis and meiosis
General Daily Schedule: 9:00-11:00 AM Lecture/Discussion
11:00-12:00 noon Lab prep; individual help
12:00-1:00 PM Lunch; individual help
1:00-3:00 PM Laboratory
Grading: Lab reports, 6 @ 25 points each 150 pts. (40%)
Curriculum project 100 pts. (27%)
Exam 100 pts. (27%)
Sharing and participation 25 pts. (6%)
Tentative scheudule of topics:
Mon. 7/7 (KK)
Lecture: Introductions, Expectations, Overview of Biotechnology
Cell structure and chromosomes
Lab: Chromosome analysis
Tues. 7/8 (KK)
Lecture: DNA structure and function
Lab: DNA isolation methods
Wed. 7/9 (KK)
Lecture: DNA cloning and analysis techniques
Lab: Biotech and the environment; Isolating soil bacteria
á extraction, dilution, and plating of soil samples on starch agar
á detection of amylase activity on Thurs.
Work on curriculum projects
Thurs. 7/10 (KK)
Lecture: DNA cloning and analysis techniques
Lab: Transformation of bacteria by plasmid containing green fluorescent protein gene
Fri. 7/11 (SB)
Lecture: Food biotechnology
á Fermentation and leavening
á Meiotic and mitotic cell cycles
á Budding yeast life cycle
Lab: Making sourdough bread
á biological vs. chemical leavening agents
á mixed vs. single cultures
o Sterile technique
o Plate yeast for next week
á how to properly pour beer containing yeast sediment
Mon. 7/14 (SB)
Lecture: Genotypes and phenotypes
á alleles - why is one yeast patch red and the other white?
á dominance - what happens when the two yeast mate?
á some phenotypes only detected under special conditions:
o Nutritional mutations
o Drug resistance
Lab: Yeast sex Ð determining mating type
á observe mating yeast with microscope
á mate with your neighbors (yeast)
o Replica plating
o Nutritional selection
Tues. 7/15 (SB)
Lecture: Reproductive biotechnology
á germ cells vs. somatic cells
á stem cells
á in vitro fertilization
o Agriculture
o Zoos
o humans
á cloning
Lab: in vitro fertilization of Xenopus eggs
á observe isolated sperm and eggs
á experiment with sperm storage conditions
o compare motilitiy
o compare fertility
Wed. 7/16 (SB)
Lecture: PCR; fundamentals and applications
á the basic idea
o discovery
o reagents required
á applications
o diagnosing genetic disease
o forensics (CSI)
o paternity testing
á most applications focus on regions where DNA sequence differs
o mutations
o polymorphisms
á detecting differences in DNA sequence
o electrophoresis
o sequencing
Lab: human and dog cheek cell PCR
á isolating template DNA
á assembling the reaction
á thermocycling (automated vs. manual)
Thurs. 7/17 (KK)
Lab: Electrophoresis of PCR products
Lab: Shoestring Biotechnology
Work on curriculum projects
Fri. 7/18 (KK)
Lecture: Bioinformatics and the Human Genome project
Lab: Case It! computer simulations of genetic testing case studies
Mon. 7/21 (KK)
Lecture: Protein purification methods
Lab: Isolation of GFP protein from transformed bacteria
Tues. 7/22 (KK)
Lecture: Antibodies and applied immunology
Lab: Diagnostic test using antibodies
Wed. 7/23 (TB)
Lecture: Genetic engineering of plants
Lab: Plant tissue culture
Work on curriculum projects
Thurs. 7/24
Fri. 7/25